《植物生理学报》 2021, 57(2): 235-247

芍药和牡丹的组织培养及遗传转化体系构建

张润龙，王小斌，邵灵梅，李丹青，夏宜平^*，张佳平^*

浙江大学农业与生物技术学院园林研究所, 观赏植物基因组与基因工程实验室, 杭州310058

通信作者：夏宜平；E-mail: ypxia@zju.edu.cn；张佳平；E-mail: zhangjiaping0604@aliyun.com

摘　要：

摘要: 芍药(Paeonia lactiflora)和牡丹(P. suffruticosa)的组织培养技术经过了多年的发展, 至今仍存在着褐化、玻璃化和污染严重, 以及分化困难、再生效率不高、生根难等问题。本篇综述论述了芍药和牡丹组织培养体系构建的研究进展, 同时探讨了它们的遗传转化体系研究现状, 这有助于全面了解芍药和牡丹的组织培养进展, 并为将来构建其稳定、高效的过表达和敲除/沉默体系提供参考。

关键词：芍药; 牡丹; 组织培养; 高效繁育; 遗传转化体系; 基因功能研究

收稿：2020-10-20   修定：2020-12-30

资助：国家自然科学基金(31600567, 32071822)和浙江省农业重 大技术协同推广计划(2018XTTGHH01)

Tissue culture and genetic transformation system construction of Paeonia lactiflora and Paeonia suffruticosa

ZHANG Runlong, WANG Xiaobin, SHAO Lingmei, LI Danqing, XIA Yiping^*, ZHANG Jiaping^*

Genomics and Genetic Engineering Laboratory of Ornamental Plants, Institute of Landscape Architecture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Corresponding author: XIA Yiping; E-mail: ypxia@zju.edu.cn; ZHANG Jiaping; E-mail: zhangjiaping0604@aliyun.com

Abstract:

After years of development, there are still some problems in tissue culture process of P. lactiflora and P. suffruticosa, such as browning, vitrifcation, serious pollution, difcult differentiation, low regeneration efciency, difcult rooting and so on. To facilitate the establishment of an efcient tissue culture system, this review summarizes the research progress in the construction of tissue culture system of P. lactiflora and P. suffruticosa, and discusses the research status of their genetic transformation system, which is helpful to fully understand the tissue culture progress of these two traditional famous flowers. It also provides a reference for the construction of stable and efcient overexpression and knockout/silencing system of P. lactiflora and P. suffruticosa in the future.

Key words: Paeonia lactiflora; Paeonia suffruticosa; tissue culture; efcient propagation; genetic transformation system; gene functional study